薬学部
Pharmacy Pharmacy

Analytical Chemistry (Tanaka Lab.)

Staff

Prof.Dr. Yoshiyuki Tanaka

Dr. Masayuki Morikawa

Research project

We are studying the mechanism of action of gene-repair enzymes and catalytic RNAs with NMR spectroscopy and enzymological analyses. Regarding the gene-repair enzyme, human 8-oxoguanine glycosylase (hOGG1) has been studied since its loss of function is deeply related with cancer. Next, regarding catalytic RNAs, hammerhead ribozyme has been studied to explore the chemical origin of life. Now, the chemical pathways of proteineous and RNA enzymes are underway.

Publications

  1. Takenori Dairaku,* Rika Kawai, Teppei Kanaba, Tetsuya Ono, Kentaro Yoshida, Hajime Sato, Kanako Nozawa-Kumada, Yoshinori Kondo, Jiro Kondo, Akira Ono, Yoshiyuki Tanaka* and Yoshitomo Kashiwagi* Effect of cytosine–Ag+–cytosine base pairing on the redox potential of the Ag+/Ag couple and the chemical reduction of Ag+ to Ag by tetrathiafulvalene. Dalton Transactions, 50, 7633-7639 (2021). (DOI: 10.1039/d1dt00975c)
  2. Jiro Kondo,* Yoshinari Tada, Takenori Dairaku, Yoshikazu Hattori, Hisao Saneyoshi, Akira Ono and Yoshiyuki Tanaka, A metallo-DNA nanowire with uninterrupted one-dimensional silver array. Nature Chemistry, 9, 956-960 (2017). (DOI: 10.1038/NCHEM.2808)
  3. Jakub Šebera, Yoshikazu Hattori, Daichi Sato, David Řeha, Radim Nencka, Takashi Kohno, Chojiro Kojima,* Yoshiyuki Tanaka,* and Vladimír Sychrovský,* The mechanism of the glycosylase reaction with hOGG1 base-excision repair enzyme: Concerted effect of Lys249 and Asp268 during excision of 8-oxoguanine. Nucleic Acids Research, 45, 5231-5242 (2017). (DOI: 1093/nar/gkx157)
  4. Hiroshi Yamaguchi#, Jakub Šebera#, Jiro Kondo#, Shuji Oda, Tomoyuki Komuro, Takuya Kawamura, Takenori Daraku, Yoshinori Kondo, Itaru Okamoto, Akira Ono, Jaroslav V. Burda, Chojiro Kojima, Vladimír Sychrovský*, Yoshiyuki Tanaka*, The structure of metallo-DNA with consecutive thymine-HgII-thymine base pairs explains positive entropy for the metallo base pair formation.Nucleic Acids Research, 42, 4094-4099 (2014). (DOI: 1093/nar/gkt1344)
  5. Yoshiyuki Tanaka*, Shuji Oda, Hiroshi Yamaguchi, Yoshinori Kondo, Chojiro Kojima and Akira Ono*, 15N-15N J-coupling across HgII: Direct observation of HgII-mediated T-T base pairs in a DNA duplex. Journal of the American Chemical Society, 129, 244-245 (2007). (DOI: 10.1021/ja065552h)
  6. Yoko Miyake, Humika Togashi, Mitsuru Tashiro, Hiroshi Yamaguchi, Shuji Oda, Megumi Kudo, Yoshiyuki Tanaka*, Yoshinori Kondo, Ryuichi Sawa, Takashi Fujimoto, Tomoya Machinami and Akira Ono*, MercuryII-mediated formation of thymine-HgII-thymine base pairs in DNA duplexes. Journal of the American Chemical Society, 128, 2172-2173 (2006). (DOI: 10.1021/ja056354d)
  7. Yoshiyuki Tanaka*, Yasuhiro Kasai, Shunsuke Mochizuki, Akihiro Wakisaka, Eugene H. Morita, Chojiro Kojima, Atsushi Toyozawa, Yoshinori Kondo, Masumi Taki, Yasuomi Takagi, Atsushi Inoue, Kazuhiko Yamasaki and Kazunari Taira*, Nature of the chemical bond formed with the structural metal ion at the A9/G10.1 motif derived from hammerhead ribozymes. Journal of the American Chemical Society, 126, 744-752 (2004). (DOI: 10.1021/ja036826t)
  8. Yoshiyuki Tanaka, Chojiro Kojima, Eugene H. Morita, Yasuhiro Kasai, Kazuhiko Yamasaki, Akira Ono, Masatsune Kainosho, and Kazunari Taira*, Identification of the Metal Ion Binding Site on an RNA Motif from Hammerhead Ribozyme using 15N-NMR Spectroscopy. Journal of the American Chemical Society, 124, 4595-4601 (2002). (DOI: 10.1021/ja011520c)
  9. Yoshiyuki Tanaka, Eugene H. Morita, Hidenori Hayashi, Yasuhiro Kasai, Toshiyuki Tanaka, and Kazunari Taira*, Well-conserved tandem G-A pairs and the flanking C-G pair in hammerhead ribozymes are sufficient for capture of structurally and catalytically important metal ions. Journal of the American Chemical Society, 122, 11303-11310 (2000). (DOI: 10.1021/ja001001u)

Review articles /Books

  1. Yoshiyuki Tanaka,* Jiro Kondo, Vladimír Sychrovský, Jakub Šebera, Takenori Dairaku, Hisao Saneyoshi, Hidehito Urata, Hidetaka Torigoe and Akira Ono,* Structures, physicochemical properties, and applications of T–HgII–T, C–AgI–C, and other metallo-base-pairs. Chemical Communications, 51, 17343-17360 (2015). (DOI: 10.1039/C5CC02693H)
  2. Akira Ono*, Hidetaka Torigoe, Yoshiyuki Tanaka and Itaru Okamoto, Binding of metal ions by pyrimidine base pairs in DNA duplexes. Chemical Society Reviews, 40, 5855-5866 (2011). DOI: 10.1039/c1cs15149e